Comparison of C4d Immunostaining Methods in Renal Allograft Biopsies
نویسندگان
چکیده
منابع مشابه
Comparison of C4d immunostaining methods in renal allograft biopsies.
Immunostaining of renal allograft biopsies for C4d deposition has become an important diagnostic tool in the recognition of humoral-mediated graft rejection. The majority of studies have been performed on frozen tissue sections with one of several commercially available antibody reagents. However, only a single small series that compared reagents or methods, including staining of formalin-fixed...
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BACKGROUND C3d is a product of both the classic and the alternative complement cascades; however, few studies have addressed the role of C3d in renal biopsies and its relationship with long-term graft survival rate is not very clear. METHODS 94 patients with biopsy-proven acute rejection episodes were included in the study. We investigated the associations between histological findings, clini...
متن کاملC4d staining in renal allograft biopsies with early acute rejection and subsequent clinical outcome.
BACKGROUND AND OBJECTIVES Diffuse C4d staining in peritubular capillaries (PTCs) during an acute rejection episode (ARE) is the footprint of antibody-mediated rejection. In current clinical practice, diffuse C4d+ staining during acute rejection is regarded as an inferior prognostic sign. This case-control study investigated the prognostic role of mere C4d staining for graft outcome during an AR...
متن کاملThrombotic microangiopathy and peritubular capillary C4d expression in renal allograft biopsies.
BACKGROUND AND OBJECTIVES This study characterizes the pathologic and clinical relationships of thrombotic microangiopathy (TMA) to antibody-mediated rejection (AMR) in renal allograft biopsies. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS Consecutive renal allograft biopsies, routinely stained for C4d over a period of 51 months (n=1101), were reviewed. For comparative analysis of histology ...
متن کاملC4d staining of renal allograft biopsies: a comparative analysis of different staining techniques.
BACKGROUND Detection of C4d along peritubular capillaries (PTC) in renal allograft biopsies is an independent prognostic marker of poor long-term graft survival. It is typically associated with circulating donor-specific antibodies. Since only little information is available on the best technique to stain C4d, we compared the two methods most often used for detecting C4d in renal allograft spec...
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ژورنال
عنوان ژورنال: Clinical Journal of the American Society of Nephrology
سال: 2006
ISSN: 1555-9041,1555-905X
DOI: 10.2215/cjn.00900805